POU1F1 antibody

Catalog No. ABIN7861945

The Mouse Monoclonal anti-POU1F1 antibody (Clone PIT1-7262) (ABIN7861945) specifically detects POU1F1 in IHC. The antibody is reactive with Human samples.

Quick Overview for POU1F1 antibody (ABIN7861945)

Target: POU1F1 (POU Class 1 Homeobox 1 (POU1F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POU1F1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: PIT1-7262

Product Details anti-POU1F1 Antibody

Purpose: POU1F1 / PIT-1 Antibody

Specificity: Transcriptional regulators play a critical role in development by mediating tissue- and cell-specific transcription. POU domain factors are transcriptional regulators characterized by a bipartite DNA binding domain, which consists of two highly conserved regions, tethered by a variable linker of 14-26 amino acids. Pit-1, also known as growth hormone factor-1 (GHF-1), a member of the POU homeodomain family, is essential for the normal development of the anterior pituitary gland, where it is required for the formation of somatotropes, lactotropes and thyrotropes. In somatotropes and lactotropes, Pit-1 activates the production of growth hormone and Prolactin, respectively. In addition, Pit-1 acts as a repressor of gene expression, which allows for the differentiation of specific cell types. Pit-1 is expressed as two alternatively spliced products, designated Pit-1a and Pit-1b, which differ in their trans-activation ability. Mutations in the Pit-1 gene are believed to result in combined pituitary hormone deficiency (CPHD) for growth hormone, Prolactin and thyroid stimulating hormone. The gene which encodes Pit-1 maps to human chromosome 3p11.2.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length POU1F1 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for POU1F1

Target: POU1F1 (POU Class 1 Homeobox 1 (POU1F1))

Alternative Name: POU1F1

Background:

Dwarf, GHF1A, Growth hormone factor 1, Hmp1, PiT-1, Pit1 beta, PIT1Z, Pituitary growth hormone, Pituitary specific positive transcription factor 1, POU domain class 1 transcription factor 1, POU domain transcriptional regulator, POU1F1

Human pituitary or tonsil.

Molecular Weight: 31-35kDa

Gene ID: 5449, 591654

UniProt: P28069

Pathways: Regulation of Carbohydrate Metabolic Process