ISL1 antibody (N-Term)

Catalog No. ABIN2779574

The Rabbit Polyclonal anti-ISL1 antibody (ABIN2779574) specifically detects ISL1 in WB. The antibody is reactive with Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Rabbit, Horse and Sheep samples.

Quick Overview for ISL1 antibody (N-Term) (ABIN2779574)

Target: ISL1 (ISL LIM Homeobox 1 (ISL1))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Rabbit, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ISL1 Antibody

Binding Specificity: N-Term

Purpose: ISL1 Antibody - N-terminal region

Sequence: IECFRCVACS RQLIPGDEFA LREDGLFCRA DHDVVERASL GAGDPLSPLH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against ISL1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ISL1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32336-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ISL1

Target: ISL1 (ISL LIM Homeobox 1 (ISL1))

Alternative Name: ISL1

Background:

Background Information: ISL1 is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. ISL1 is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: ISL LIM homeobox 1

Alternative Symbols: Isl-1, ISLET1

Protein Name: Insulin gene enhancer protein ISL-1

Molecular Weight: 39kDa

Gene ID: 3670

NCBI Accession: NP_002193

UniProt: P61371

Pathways: Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding