TLE2 antibody (N-Term)

Catalog No. ABIN2779582

The Rabbit Polyclonal anti-TLE2 antibody has been validated for WB. It is suitable to detect TLE2 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig and Pig.

Quick Overview for TLE2 antibody (N-Term) (ABIN2779582)

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLE2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TLE2 Antibody

Binding Specificity: N-Term

Sequence: VEEERPSGPG GGGKQRADEK EPSGPYESDE DKSDYNLVVD EDQPSEPPSP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 85%, Pig: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TLE2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TLE2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 743 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TLE2

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Alternative Name: TLE2

Background: TLE2 belongs to the WD repeat Groucho/TLE family. It contains 6 WD repeats. TLE2 is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
Alias Symbols: ESG, ESG2, FLJ41188, GRG2
Protein Interaction Partner: UBC, SUMO1, ORF50, LEF1, BARHL1, TLX3, VENTX, CUL4A, CUL4B, PAX9, MSX2, MSX1, FOXA3, EMX2, PRDM1, H3F3B, TLE1, UTY, HMGB1, RUNX1, TLE2, HES1,
Protein Size: 743

Molecular Weight: 80 kDa

Gene ID: 7089

NCBI Accession: NM_003260, NP_003251

UniProt: Q04725

Pathways: WNT Signaling