TLE2 antibody (AA 180-260)

Catalog No. ABIN7224714

The Rabbit Polyclonal anti-TLE2 antibody is suitable to detect TLE2 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for TLE2 antibody (AA 180-260) (ABIN7224714)

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TLE2 Antibody

Binding Specificity: AA 180-260

Purpose: Rabbit Anti-TLE2 Polyclonal Antibody

Specificity: TLE2 Polyclonal Antibody detects endogenous levels of TLE2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human TLE2 at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TLE2

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Alternative Name: TLE2

Background: TLE2, Transducin-like enhancer protein 2, Enhancer of split groucho-like protein 2, ESG2Transducin-like enhancer of split proteins (TLE1, TLE2, TLE3, TLE4, and TLE6) are mammalian homologs of Drosophila Groucho. TLEs contain several WD-repeats implicated in protein-protein interaction. TLEs are transcriptional co-repressors that bind to many transcription factors such as LEF1, Runx1, Oct-1, hepatocyte nuclear factor 3-β as well as histone H3. TLEs are differentially expressed during animal development and may have overlapping as well as distinct functions.

Molecular Weight: 110kD

Gene ID: 7089

UniProt: Q04725

Pathways: WNT Signaling