ASCL1 antibody (N-Term)

Catalog No. ABIN2779585

This Rabbit Polyclonal antibody specifically detects ASCL1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Dog and Rabbit and has been mentioned in 2+ publications.

Quick Overview for ASCL1 antibody (N-Term) (ABIN2779585)

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ASCL1 Antibody

Binding Specificity: N-Term

Sequence: QSAQQQQQQQ QQQQQAPQLR PAADGQPSGG GHKSAPKQVK RQRSSSPELM

Predicted Reactivity: Cow: 100%, Dog: 92%, Guinea Pig: 100%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against ASCL1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ASCL1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 236 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ASCL1 antibody (ABIN2779585)

Suozzi, Malatesta, Zancanaro: "Subcellular distribution of key enzymes of lipid metabolism during the euthermia-hibernation-arousal cycle." in: Journal of anatomy, Vol. 214, Issue 6, pp. 956-62, (2009) (PubMed).

Vias, Massie, East, Scott, Warren, Zhou, Nikitin, Neal, Mills: "Pro-neural transcription factors as cancer markers." in: BMC medical genomics, Vol. 1, pp. 17, (2008) (PubMed).

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: ASCL1

Background: ASCL1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. It is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers.
Alias Symbols: ASH1, HASH1, MASH1, bHLHa46
Protein Interaction Partner: MEF2D, MEF2C, MEF2A, NEUROG2, UBQLN1, EP300, TCF4, BMP2, ASCL1,
Protein Size: 236

Molecular Weight: 25 kDa

Gene ID: 429

NCBI Accession: NM_004316, NP_004307

UniProt: P50553

Pathways: Dopaminergic Neurogenesis