TLE4 antibody (N-Term)

Catalog No. ABIN486927

The Rabbit Polyclonal anti-TLE4 antibody (ABIN486927) specifically detects TLE4 in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse and Rabbit samples.

Quick Overview for TLE4 antibody (N-Term) (ABIN486927)

Target: TLE4 (Transducin-like Enhancer Protein 4 (TLE4))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLE4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TLE4 Antibody

Binding Specificity: N-Term

Purpose: TLE4 Antibody - N-terminal region

Sequence: GAEKHRNSAD YSSESKKQKT EEKEIAARYD SDGEKSDDNL VVDVSNEDPS

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TLE4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TLE4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32381-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TLE4

Target: TLE4 (Transducin-like Enhancer Protein 4 (TLE4))

Alternative Name: TLE4

Background:

Background Information: TLE4 is the transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.

Gene Name: Transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)

Alternative Symbols: ESG, BCE1, ESG4, GRG4, BCE-1, Grg-4, E(spI), E(spl)

Protein Name: Transducin-like enhancer protein 4

Molecular Weight: 84kDa

Gene ID: 7091

NCBI Accession: NP_008936

UniProt: Q04727

Pathways: WNT Signaling