SIRT2 antibody (N-Term)

Catalog No. ABIN2779604

Product Details anti-SIRT2 Antibody

Target: SIRT2 (Sirtuin 2 (SIRT2))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Rabbit, Dog, Guinea Pig, Cow, Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MAEPDPSHPL ETQAGKVQEA QDSDSDSEGG AAGGEADMDF LRNLFSQTLS

Predicted Reactivity: Cow: 92%, Dog: 82%, Guinea Pig: 85%, Horse: 92%, Human: 100%, Mouse: 92%, Pig: 92%, Rabbit: 100%, Rat: 77%, Yeast: 79%

Characteristics: This is a rabbit polyclonal antibody against SIRT2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SIRT2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 389 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SIRT2 antibody (ABIN2779604)

Benavente, Schnell, Jacobson: "Effects of niacin restriction on sirtuin and PARP responses to photodamage in human skin." in: PLoS ONE, Vol. 7, Issue 7, pp. e42276, (2012) (PubMed).

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2 (SIRT2 Products)

Background: SIRT2 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by SIRT2 is included in class I of the sirtuin family.
Alias Symbols: SIR2, SIR2L, SIR2L2
Protein Interaction Partner: ACLY, SIRT1, UBC, ING1, EP300, CBLB, CBL, XPO1, CDC20, FZR1, RUNX3, PCK1, FOXO1, HDAC6, tat, RBP1, HIST1H3A, NUDT21, PAPOLA, DNAJB8, DNAJB6, SP140, KAT2B, CDC14B, KAT2A, TUBA4A, HOXA10, TUBB, KRT78, RTN4, PHB2, SPOP, ELL, SARS, GYS1,
Protein Size: 389

Molecular Weight: 43 kDa

Gene ID: 22933

NCBI Accession: NM_012237, NP_036369

UniProt: Q8IXJ6