SIRT3 antibody (C-Term)

Catalog No. ABIN2779610

This Rabbit Polyclonal antibody specifically detects SIRT3 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Horse and Cow and has been mentioned in 3+ publications.

Quick Overview for SIRT3 antibody (C-Term) (ABIN2779610)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SIRT3 Antibody

Binding Specificity: C-Term

Sequence: VGPLAWHPRS RDVAQLGDVV HGVESLVELL GWTEEMRDLV QRETGKLDGP

Predicted Reactivity: Cow: 100%, Dog: 79%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against SIRT3. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SIRT3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 257 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SIRT3 antibody (ABIN2779610)

Vidimar, Gius, Chakravarti, Bulun, Wei, Kim: "Dysfunctional MnSOD leads to redox dysregulation and activation of prosurvival AKT signaling in uterine leiomyomas." in: Science advances, Vol. 2, Issue 11, pp. e1601132, (2016) (PubMed).

Marfè, Tafani, Fiorito, Pagnini, Iovane, De Martino: "Involvement of FOXO transcription factors, TRAIL-FasL/Fas, and sirtuin proteins family in canine coronavirus type II-induced apoptosis." in: PLoS ONE, Vol. 6, Issue 11, pp. e27313, (2011) (PubMed).

Lanza, Short, Short, Raghavakaimal, Basu, Joyner, McConnell, Nair: "Endurance exercise as a countermeasure for aging." in: Diabetes, Vol. 57, Issue 11, pp. 2933-42, (2008) (PubMed).

Target Details for SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: SIRT3

Background: The SIRT3 gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. SIRT3 encodes a protein that is included in class I of the sirtuin family.
Alias Symbols: SIR2L3
Protein Interaction Partner: SIRT5, SKP2, UBC, HSPD1, CMYA5, PGAP1, RIF1, NCAPD2, HERC2, TTF2, UQCRH, UGDH, HSP90B1, SNTB1, SBF1, HSPA9, HSPA5, HSPA4, HSPA1L, GRN, ATP5B, ATP5A1, SIRT4, tat, XRCC6, FOXO3, DNM1L, PEX5,
Protein Size: 257

Molecular Weight: 28 kDa

Gene ID: 23410

NCBI Accession: NM_001017524, NP_001017524

UniProt: Q9NTG7