ZEB1 antibody (N-Term)

Catalog No. ABIN2779631

There is 1 publication for this product available. The Rabbit Polyclonal anti-ZEB1 antibody is suitable to detect ZEB1 in samples from Human, Mouse, Rat, Dog, Rabbit, Cow and Guinea Pig. It has been validated for WB and IF.

Quick Overview for ZEB1 antibody (N-Term) (ABIN2779631)

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ZEB1 Antibody

Binding Specificity: N-Term

Purpose: ZEB1 Antibody - N-terminal region

Sequence: KDDECESDAE NEQNHDPNVE EFLQQQDTAV IFPEAPEEDQ RQGTPEASGH

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ZEB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ZEB1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32422-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ZEB1 antibody (ABIN2779631)

Lobert, Graichen, Morris: "Coordinated regulation of ?-tubulin isotypes and epithelial-to-mesenchymal transition protein ZEB1 in breast cancer cells." in: Biochemistry, Vol. 52, Issue 32, pp. 5482-90, (2013) (PubMed).

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1

Background:

Background Information: ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM].

Gene Name: Zinc finger E-box binding homeobox 1

Alternative Symbols: BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1

Protein Name: Zinc finger E-box-binding homeobox 1

Molecular Weight: 124kDa

Gene ID: 6935

NCBI Accession: NP_110378

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation