OTX2 antibody (N-Term)

Catalog No. ABIN2779643

Cited in 3+ publications. The Rabbit Polyclonal anti-OTX2 antibody (ABIN2779643) specifically detects OTX2 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Sheep, Rabbit, Horse, Cow and Guinea Pig samples.

Quick Overview for OTX2 antibody (N-Term) (ABIN2779643)

Target: OTX2 (Orthodenticle Homeobox 2 (OTX2))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Sheep, Rabbit, Horse, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OTX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-OTX2 Antibody

Binding Specificity: N-Term

Purpose: OTX2 Antibody - N-terminal region

Sequence: PESRVQVWFK NRRAKCRQQQ QQQQNGGQNK VRPAKKKTSP AREVSSESGT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against OTX2. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human OTX2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32439-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-OTX2 antibody (ABIN2779643)

Machali?ska, Kawa, Pius-Sadowska, Rogi?ska, K?os, Baumert, Wiszniewska, Machali?ski: "Endogenous regeneration of damaged retinal pigment epithelium following low dose sodium iodate administration: an insight into the role of glial cells in retinal repair." in: Experimental eye research, Vol. 112, pp. 68-78, (2013) (PubMed).

Aluigi, Angelini, Corte, Falugi: "The sea urchin, Paracentrotus lividus, embryo as a "bioethical" model for neurodevelopmental toxicity testing: effects of diazinon on the intracellular distribution of OTX2-like proteins." in: Cell biology and toxicology, Vol. 24, Issue 6, pp. 587-601, (2008) (PubMed).

Takeda, Yokoyama, Yasumoto, Saito, Udono, Takahashi, Shibahara: "OTX2 regulates expression of DOPAchrome tautomerase in human retinal pigment epithelium." in: Biochemical and biophysical research communications, Vol. 300, Issue 4, pp. 908-14, (2003) (PubMed).

Target Details for OTX2

Target: OTX2 (Orthodenticle Homeobox 2 (OTX2))

Alternative Name: OTX2

Background:

Background Information: Homeobox protein OTX2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. This protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants may exist, but their full length sequences have not been determined. This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants may exist, but their full length sequences have not been determined.

Gene Name: Orthodenticle homeobox 2

Alternative Symbols: CPHD6, MCOPS5

Protein Name: Homeobox protein OTX2

Molecular Weight: 32kDa

Gene ID: 5015

NCBI Accession: NP_068374

UniProt: P32243

Pathways: Dopaminergic Neurogenesis