HIPK2 antibody (Middle Region)

Catalog No. ABIN2779735

The Rabbit Polyclonal anti-HIPK2 antibody has been validated for WB and IHC. It is suitable to detect HIPK2 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). There are 3+ publications available.

Quick Overview for HIPK2 antibody (Middle Region) (ABIN2779735)

Target: HIPK2 (Homeodomain Interacting Protein Kinase 2 (HIPK2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIPK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HIPK2 Antibody

Binding Specificity: Middle Region

Sequence: MWSLGCVIAE LFLGWPLYPG ASEYDQIRYI SQTQGLPAEY LLSAGTKTTR

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 87%

Characteristics: This is a rabbit polyclonal antibody against HIPK2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human HIPK2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1198 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HIPK2 antibody (ABIN2779735)

Upadhyay, Bhadauriya, Ganesh: "Heat shock modulates the subcellular localization, stability, and activity of HIPK2." in: Biochemical and biophysical research communications, Vol. 472, Issue 4, pp. 580-4, (2016) (PubMed).

Steinmann, Schulte, Beck, Chachra, Bujnicki, Klempnauer: "v-Myc inhibits C/EBPbeta activity by preventing C/EBPbeta-induced phosphorylation of the co-activator p300." in: Oncogene, Vol. 28, Issue 26, pp. 2446-55, (2009) (PubMed).

Choi, Seo, Kim, Sung, Ahn, Park, Lee, Choi: "Ubiquitination and degradation of homeodomain-interacting protein kinase 2 by WD40 repeat/SOCS box protein WSB-1." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4682-9, (2008) (PubMed).

Target Details for HIPK2

Target: HIPK2 (Homeodomain Interacting Protein Kinase 2 (HIPK2))

Alternative Name: HIPK2

Background: HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.
Alias Symbols: DKFZp686K02111, FLJ23711, PRO0593
Protein Interaction Partner: UBC, TP53, SIAH2, XAF1, AATF, SUMO1, MDM2, AXIN1, SIAH1, MBP, DAXX, MKNK1, MECP2, ZBTB4, HIPK2, DCAF7, DYRK1B, MAP3K1, DYRK1A, FBXO3, CBX4, SUMO2, SUMO3, PML, PAX6, CHMP4B, SENP2, SENP1, UBE2I, HIST2H2BE, CXCL1, SIAH1P1, WSB1, MYBL2, MYBL1, NLK, MAP3K7, M
Protein Size: 1198

Molecular Weight: 120 kDa

Gene ID: 28996

NCBI Accession: NM_022740, NP_073577

UniProt: Q9H2X6

Pathways: Cell Division Cycle