SMAD4 antibody (Middle Region)

Catalog No. ABIN2779802

The Rabbit Polyclonal anti-SMAD4 antibody (ABIN2779802) specifically detects SMAD4 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Rabbit, Goat, Guinea Pig, Horse and Sheep samples.

Quick Overview for SMAD4 antibody (Middle Region) (ABIN2779802)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Rabbit, Goat, Guinea Pig, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD4 Antibody

Binding Specificity: Middle Region

Sequence: YVHDFEGQPS LPTEGHSIQT IQHPPSNRAS TETYSAPALL APAESNATST

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against Smad4. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human Smad4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 551 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: Smad4

Background: The function of Smad4 remains unknown.
Alias Symbols: AW743858, D18Wsu70e, DPC4, Madh4
Protein Interaction Partner: Smad2, Smad3, Prdm16, Ddx5, Col1a1, Col5a1, Col3a1, Sf3b2, Pex6, Zmym2, Eif4enif1, Pknox1, Pbx1, Smad4, Sumo1, Lef1, tob1a, Smarcd3, Apc, Gata6, Gata5, Gata4, Foxp1, Tlx3, Snai1, Hdac1, Sin3a, Cdkn2b, Tgfb1, Tob1,
Protein Size: 551

Molecular Weight: 60 kDa

Gene ID: 17128

NCBI Accession: NM_008540, NP_032566

UniProt: P97471

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy