DNMT1 antibody (N-Term)

Catalog No. ABIN2779926

The Rabbit Polyclonal anti-DNMT1 antibody has been validated for WB. It is suitable to detect DNMT1 in samples from Mouse.

Quick Overview for DNMT1 antibody (N-Term) (ABIN2779926)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DNMT1 Antibody

Binding Specificity: N-Term

Sequence: SSVATRRTTR QTTITAHFTK GPTKRKPKEE SEEGNSAESA AEERDQDKKR

Predicted Reactivity: Mouse: 100%

Characteristics: This is a rabbit polyclonal antibody against Dnmt1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of mouse Dnmt1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Antigen size: 1619 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: Dnmt1

Background: Dnmt1 methylates CpG residues.Dnmt1 preferentially methylates hemimethylated DNA. Dnmt1 associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance.Dnmt1 associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones.Dnmt1 mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, Dnmt1 is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Alias Symbols: Cxxc9, Dnmt, Dnmt1o, MTase, Met-1, Met1, MommeD2, MCMT, m.MmuI
Protein Interaction Partner: Uhrf1, Parp1, Eed, hist1h3g, DSK2, VPS9, Kat5, Ubc, Atm, NUB1, UBQLN1, SQSTM1, PSMD4, NBR1, RAD23B, Hesx1, Ezh2, Zbtb16, Cul3, Cdh1, Dmap1, Tcf3, hist1h4a, hist1h2bj, hist2h2ab, Dnmt1, Kcnq1ot1, Pla2g2a, Apc, Tox3, Rela, HDAC1,
Protein Size: 1619

Molecular Weight: 183 kDa

Gene ID: 13433

NCBI Accession: NM_010066, NP_034196

UniProt: P13864

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection