MCAF1 antibody (Middle Region)

Catalog No. ABIN2780013

The Rabbit Polyclonal anti-MCAF1 antibody (ABIN2780013) specifically detects MCAF1 in WB. The antibody is reactive with Mouse, Pig and Rat samples.

Quick Overview for MCAF1 antibody (Middle Region) (ABIN2780013)

Target: MCAF1 (ATF7IP) (Activating Transcription Factor 7 Interacting Protein (ATF7IP))

Reactivity: Mouse, Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCAF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCAF1 Antibody

Binding Specificity: Middle Region

Purpose: Atf7ip Antibody - middle region

Sequence: MSTPEGEKSE KDGKAEEEER VPAEEQPPVR NEFSRRKRSK SEDMDSVESK

Predicted Reactivity: Mouse: 100%, Pig: 92%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against Atf7ip. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Mouse Atf7ip

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP37246-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MCAF1

Target: MCAF1 (ATF7IP) (Activating Transcription Factor 7 Interacting Protein (ATF7IP))

Alternative Name: Atf7ip

Background:

Background Information: Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Atf7ip can both act as an activator or a repressor depending on the context. It mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Atf7ip is required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). Atf7ip may have ATPase activity.

Gene Name: activating transcription factor 7 interacting protein

Alternative Symbols: A, AM, Mcaf, Mcaf1, 2610204M12Rik, 5830415B17Rik

Protein Name: Activating transcription factor 7-interacting protein 1

Molecular Weight: 138kDa

Gene ID: 54343

NCBI Accession: NP_062299

UniProt: Q7TT18