T-Bet antibody (C-Term)

Catalog No. ABIN2780015

This Rabbit Polyclonal antibody specifically detects T-Bet in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Pig, Rabbit and Sheep.

Quick Overview for T-Bet antibody (C-Term) (ABIN2780015)

Target: T-Bet

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Bet antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-T-Bet Antibody

Binding Specificity: C-Term

Sequence: MDPGLGSSEE QGSSPSLWPE VTSLQPESSD SGLGEGDTKR RRISPYPSSG

Predicted Reactivity: Cow: 86%, Dog: 86%, Human: 93%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 93%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against TBX21. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of mouse TBX21

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 530 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for T-Bet

Target: T-Bet

Alternative Name: TBX21

Background: TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. It is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
Alias Symbols: TBT1, Tbet, Tblym
Protein Interaction Partner: Bcl6, Smarca4, Tlx3, Tead4, Pax9, Gsc, Gata3, Dlx1, Alx4, Rbpj, Stat1, Ifng, Tec, Itk, Txk,
Protein Size: 530

Molecular Weight: 58 kDa

Gene ID: 57765

NCBI Accession: NM_019507, NP_062380

UniProt: Q9JKD8