PUS1 antibody (C-Term)

Catalog No. ABIN2780021

The Rabbit Polyclonal anti-PUS1 antibody (ABIN2780021) specifically detects PUS1 in WB. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for PUS1 antibody (C-Term) (ABIN2780021)

Target: PUS1 (Pseudouridylate Synthase 1 (PUS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PUS1 Antibody

Binding Specificity: C-Term

Purpose: PUS1 Antibody - C-terminal region

Sequence: PTIVSTERDE RSMAQWLNTL PIHNFSGTAL GAADTGAKVP SSLEGSEGDG

Predicted Reactivity: Human: 92%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PUS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109617

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PUS1

Target: PUS1 (Pseudouridylate Synthase 1 (PUS1))

Alternative Name: PUS1

Background:

Background Information: Pus1 converts specific uridines to PSI in a number of tRNA substrates. Pus1 acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Pus1 is involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA.

Gene Name: Pseudouridine synthase 1

Alternative Symbols: MPU, MPUS1, mPus1p, A730013B20Rik

Protein Name: tRNA pseudouridine synthase A, mitochondrial

Molecular Weight: 48kDa

Gene ID: 56361

NCBI Accession: NP_001020733

UniProt: Q791J1