NMRAL1 antibody (N-Term)

Catalog No. ABIN2780056

This Rabbit Polyclonal antibody specifically detects NMRAL1 in WB. It exhibits reactivity toward Rat, Mouse, Cow, Dog and Pig.

Quick Overview for NMRAL1 antibody (N-Term) (ABIN2780056)

Target: NMRAL1 (NmrA-Like Family Domain Containing 1 (NMRAL1))

Reactivity: Rat, Mouse, Cow, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMRAL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NMRAL1 Antibody

Binding Specificity: N-Term

Purpose: Nmral1 Antibody - N-terminal region

Sequence: GATGAQGGSV ARALLEDGTF RIRVVTRNPE QRAAKELKQQ GAEVVRGDQD

Predicted Reactivity: Cow: 79%, Dog: 79%, Mouse: 100%, Pig: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against Nmral1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the n terminal region of mouse Nmral1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP37361-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NMRAL1

Target: NMRAL1 (NmrA-Like Family Domain Containing 1 (NMRAL1))

Alternative Name: Nmral1

Background:

Background Information: Nmral1 is a redox sensor protein. Nmral1 undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP+ levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH.Nmral1 has higher affinity for NADPH than for NADP+. Binding to NADPH is necessary to form a stable dimer.

Gene Name: NmrA-like family domain containing 1

Alternative Symbols: AI256624, 1110025F24Rik

Protein Name: NmrA-like family domain-containing protein 1

Molecular Weight: 34kDa

Gene ID: 67824

NCBI Accession: NP_080669

UniProt: Q8K2T1