ISL2 antibody (C-Term)

Catalog No. ABIN2780062

This Rabbit Polyclonal antibody specifically detects ISL2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Horse, Guinea Pig, Rabbit and Dog.It has been mentioned in 1 publication

Quick Overview for ISL2 antibody (C-Term) (ABIN2780062)

Target: ISL2 (ISL LIM Homeobox 2 (ISL2))

Reactivity: Human, Mouse, Rat, Cow, Horse, Guinea Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISL2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ISL2 Antibody

Binding Specificity: C-Term

Purpose: ISL2 Antibody - C-terminal region

Sequence: VIRVWFQNKR CKDKKKSILM KQLQQQQHSD KASFQGLTGT PLVAGSPIGH

Predicted Reactivity: Cow: 79%, Dog: 79%, Guinea Pig: 86%, Horse: 79%, Human: 86%, Mouse: 93%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against ISL2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of mouse ISL2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8103491

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ISL2 antibody (ABIN2780062)

Svärd, Heby-Henricson, Henricson, Persson-Lek, Rozell, Lauth, Bergström, Ericson, Toftgård, Teglund: "Genetic elimination of Suppressor of fused reveals an essential repressor function in the mammalian Hedgehog signaling pathway." in: Developmental cell, Vol. 10, Issue 2, pp. 187-97, (2006) (PubMed).

Target Details for ISL2

Target: ISL2 (ISL LIM Homeobox 2 (ISL2))

Alternative Name: ISL2

Background:

Background Information: Isl2 specifies RGC laterality by repressing an ipsilateral pathfinding program unique to VTC RGCs and involving Zic2 and EphB1. This genetic hierarchy controls binocular vision.

Gene Name: Insulin related protein 2 (islet 2)

Alternative Symbols: isle, 3110001N10Rik

Protein Name: Insulin gene enhancer protein ISL-2

Molecular Weight: 39kDa

Gene ID: 104360

NCBI Accession: NP_081673

UniProt: Q9CXV0