NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (Middle Region)

Details for Product anti-NFAT5 Antibody No. ABIN2780105
Antigen
  • zgc:63502
  • wu:fi46f11
  • TonEBP
  • NFAT5
  • nfatz
  • orebp
  • nf-at5
  • nfatl1
  • tonebp
  • DKFZp469P1514
  • AI225870
  • B130038B15Rik
  • CAG-8
  • CAG80
  • NFATL1
  • OREBP
  • mKIAA0827
  • NF-AT5
  • NFATZ
  • TONEBP
  • Nfat
  • nuclear factor of activated T-cells 5
  • nuclear factor of activated T-cells 5, tonicity-responsive a
  • nuclear factor of activated T-cells 5, tonicity-responsive S homeolog
  • nuclear factor of activated T-cells 5, tonicity-responsive
  • nuclear factor of activated T cells 5
  • NFAT5
  • nfat5a
  • nfat5.S
  • nfat5
  • Nfat5
Epitope
Middle Region
20
14
8
8
6
6
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
104
80
37
3
2
2
2
1
1
1
Host
Rabbit
101
5
Clonality
Polyclonal
Conjugate
This NFAT5 antibody is un-conjugated
7
6
6
6
6
6
6
3
3
2
2
2
2
2
Application
Western Blotting (WB)
38
32
26
16
12
6
2
Options
Immunogen The immunogen is a synthetic peptide directed towards the middle region of mouse NFAT5
Blocking Peptide Blocking peptide for this product available: ABIN980636
Sequence TIQTQDISQP GTFPAVSAAS QLPSSDALLQ QATQFQTREA QSRDTIQSDT
Predicted Reactivity Cow: 93%, Dog: 100%, Guinea Pig: 83%, Horse: 93%, Human: 93%, Mouse: 100%, Pig: 93%, Rabbit: 86%, Rat: 93%
Characteristics This is a rabbit polyclonal antibody against NFAT5. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Plasmids, Primers & others Plasmids, Primers & others NFAT5 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name NFAT5 (NFAT5 Antibody Abstract)
Background Nfat5 contains 1 RHD (Rel-like) domain, and plays a role in the inducible expression of genes. Nfat5 regulates hypertonicity-induced cellular accumulation of osmolytes. Nfat5 might be capable of forming stable dimers with DNA elements
Alias Symbols: CAG-8, CAG80, OREBP, nfatz, NFATL1, TonEBP, AI225870, mKIAA0827, B130038B15Rik
Protein Size: 1534
Molecular Weight 166 kDa
Gene ID 54446
NCBI Accession NM_018823
UniProt Q9WV30
Pathways RTK Signaling, WNT Signaling
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1534 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) (Middle Region) antibody (ABIN2780105) WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posi...
Image no. 2 for anti-Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) (Middle Region) antibody (ABIN2780105) Lanes: Lane 1:241 µg HT 29 lysate blocked with 5 % FBS Lane 2: 041 µg HT 29 lysate bl...
Background publications Pan, Tsuruta, Masuda, Imamura, Bazan, Arai, Arai, Miyatake: "NFATz: a novel rel similarity domain containing protein." in: Biochemical and biophysical research communications, Vol. 272, Issue 3, pp. 765-76, 2000 (PubMed).

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