MED21 antibody (Middle Region)

Catalog No. ABIN2780233

Product Details anti-MED21 Antibody

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED21 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DSLPSEESTA ALQAASLYKL EEENHEAATC LEDVVYRGDM LLEKIQSALA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MED21. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MED21

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 144 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED21

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Alternative Name: MED21 (MED21 Products)

Background: MED21 belongs to the Mediator complex subunit 21 family. It is the component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alias Symbols: SRB7, SURB7
Protein Interaction Partner: MAGEB4, SSC5D, ZNF655, BLOC1S6, MED21, CDK8, MED19, MED26, UBC, HTT, FBXW7, MED11, MED8, MED28, MED29, MED18, MED4, MED16, MED6, MED13, MED12, MED24, MED17, MED14, MED22, MED1, ELAVL1, VAV2, SKP1, THRA, THRB, MED10, TP53, SREBF1, ZC3H13, ZSCAN1, PCBD2, TA
Protein Size: 144

Molecular Weight: 15 kDa

Gene ID: 9412

NCBI Accession: NM_004264, NP_004255

UniProt: Q13503

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha