MITF antibody (Middle Region)

Catalog No. ABIN2780334

The Rabbit Polyclonal anti-MITF antibody has been validated for WB. It is suitable to detect MITF in samples from Human, Mouse, Dog, Rat, Rabbit, Horse, Guinea Pig, Cow, Sheep, Zebrafish (Danio rerio) and Goat. There is 1 publication available.

Quick Overview for MITF antibody (Middle Region) (ABIN2780334)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human, Mouse, Dog, Rat, Rabbit, Horse, Guinea Pig, Cow, Sheep, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MITF Antibody

Binding Specificity: Middle Region

Sequence: MGSKLEDILM DDTLSPVGVT DPLLSSVSPG ASKTSSRRSS MSMEETEHTC

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MITF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MITF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 419 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MITF antibody (ABIN2780334)

Ursini, Cavalleri, Fazio, Angrisano, Iacovelli, Porcelli, Maddalena, Punzi, Mancini, Gelao, Romano, Masellis, Calabrese, Rampino, Taurisano, Di Giorgio, Keller, Tarantini, Sinibaldi, Quarto et al.: "BDNF rs6265 methylation and genotype interact on risk for schizophrenia. ..." in: Epigenetics, Vol. 11, Issue 1, pp. 11-23, (2016) (PubMed).

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background: MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alias Symbols: WS2A, MI, WS2, bHLHe32
Protein Interaction Partner: EBP, STUB1, HK3, EPAS1, LEF1, APP, HINT1, ELAVL1, CDK2, SUMO2, PSMD14, MARK3, PIAS3, PIAS2, PIAS1, SUMO1, UBE2I, PAX3, OTX2, PAX6, PATZ1, GSK3B, TFEC, EP300, RPS6KA1, TFEB, MAPK14, TFE3, MAPK1, SPI1, FOS, CTNNB1, CDKN1A, RB1,
Protein Size: 419

Molecular Weight: 47 kDa

Gene ID: 4286

NCBI Accession: NM_000248, NP_000239

Pathways: Chromatin Binding