RFX5 antibody (C-Term)

Catalog No. ABIN2780343

The Rabbit Polyclonal anti-RFX5 antibody (ABIN2780343) specifically detects RFX5 in WB. The antibody is reactive with Human, Mouse, Rat, Guinea Pig and Horse samples.

Quick Overview for RFX5 antibody (C-Term) (ABIN2780343)

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFX5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RFX5 Antibody

Binding Specificity: C-Term

Purpose: RFX5 Antibody - C-terminal region

Sequence: VIKGSRSQKE AFPLAKGEVD TAPQGNKDLK EHVLQSSLSQ EHKDPKATPP

Predicted Reactivity: Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against RFX5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RFX5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8110251

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RFX5

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Alternative Name: RFX5

Background:

Background Information: RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. RFX is a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS, MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS, MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS, MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.

Gene Name: Regulatory factor X, 5 (influences HLA class II expression)

Alternative Symbols: -

Protein Name: DNA-binding protein RFX5

Molecular Weight: 65kDa

Gene ID: 5993

NCBI Accession: NP_000440

UniProt: P48382