TWIST1 antibody (C-Term)

Catalog No. ABIN2780347

The Rabbit Polyclonal anti-TWIST1 antibody has been validated for WB and IHC. It is suitable to detect TWIST1 in samples from Human, Mouse, Rat, Cow, Horse, Zebrafish (Danio rerio), Pig, Dog, Rabbit and Guinea Pig. There is 1 publication available.

Quick Overview for TWIST1 antibody (C-Term) (ABIN2780347)

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reactivity: Human, Mouse, Rat, Cow, Horse, Zebrafish (Danio rerio), Pig, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWIST1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TWIST1 Antibody

Binding Specificity: C-Term

Sequence: DKLSKIQTLK LAARYIDFLY QVLQSDELDS KMASCSYVAH ERLSYAFSVW

Predicted Reactivity: Cow: 100%, Dog: 79%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 79%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against TWIST1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TWIST1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 202 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TWIST1 antibody (ABIN2780347)

Guha, Bandyopadhyaya, Polumuri, Chumsri, Gade, Kalvakolanu, Ahmed et al.: "Nicotine promotes apoptosis resistance of breast cancer cells and enrichment of side population cells with cancer stem cell-like properties via a signaling cascade involving galectin-3, α9 nicotinic ..." in: Breast cancer research and treatment, Vol. 145, Issue 1, pp. 5-22, (2014) (PubMed).

Target Details for TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: TWIST1

Background: Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation.TWIST1 is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue, in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
Alias Symbols: SCS, ACS3, CRS1, BPES2, BPES3, TWIST, bHLHa38
Protein Interaction Partner: UBC, HOXA5, ETS2, TP53, KAT2B, SETD8, HES6, MTA2, BRAP, TCF3, RELA, PPP2CA, HDAC2, CHD3, TWIST2, ELSPBP1, WDR5, HDAC3, RBBP7, CHD4, SOX2, EP300, TWIST1, GLI3, MYOD1,
Protein Size: 202

Molecular Weight: 21 kDa

Gene ID: 7291

NCBI Accession: NM_000474, NP_000465

UniProt: Q15672

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity