EN1 antibody (Middle Region)

Catalog No. ABIN2780360

The Rabbit Polyclonal anti-EN1 antibody is suitable to detect EN1 in samples from Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for EN1 antibody (Middle Region) (ABIN2780360)

Target: EN1 (Engrailed Homeobox 1 (EN1))

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Dog, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EN1 Antibody

Binding Specificity: Middle Region

Purpose: EN1 Antibody - middle region

Sequence: LNESQIKIWF QNKRAKIKKA TGIKNGLALH LMAQGLYNHS TTTVQDKDES

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 80%

Characteristics: This is a rabbit polyclonal antibody against EN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EN1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38029-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EN1

Target: EN1 (Engrailed Homeobox 1 (EN1))

Alternative Name: EN1

Background:

Background Information: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Differe

Gene Name: Engrailed homeobox 1

Alternative Symbols: ENDOVESLB

Protein Name: Homeobox protein engrailed-1

Molecular Weight: 40 kDa

Gene ID: 2019

NCBI Accession: NP_001417

UniProt: Q05925

Pathways: Dopaminergic Neurogenesis