EGR4 antibody (C-Term)

Catalog No. ABIN183606

The Rabbit Polyclonal anti-EGR4 antibody (ABIN183606) specifically detects EGR4 in WB. The antibody is reactive with Mouse, Human, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for EGR4 antibody (C-Term) (ABIN183606)

Target: EGR4 (Early Growth Response 4 (EGR4))

Reactivity: Mouse, Human, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EGR4 Antibody

Binding Specificity: C-Term

Purpose: EGR4 Antibody - C-terminal region

Sequence: RSDHLTSHVR THTGEKPFAC DVCGRRFARS DEKKRHSKVH LKQKARAEER

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against EGR4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EGR4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099424

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EGR4

Target: EGR4 (Early Growth Response 4 (EGR4))

Alternative Name: EGR4

Background:

Background Information: The nerve growth factor-induced clone C (NGFI-C/EGR4) gene is a zinc-finger transcription factor that is rapidly induced by nerve growth factor in rat pheochromocytoma PC12 cells and by seizure in brain. NGFI-C/EGR4 is closely related to the previously described early response genes, nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR-4 functionally cooperate with NFAT proteins and induce expression of IL-2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.

Gene Name: Early growth response 4

Alternative Symbols: AT133, NGFIC, NGFI-C, PAT133

Protein Name: Early growth response protein 4

Molecular Weight: 51kDa

Gene ID: 1961

NCBI Accession: NP_001956

UniProt: Q05215