FLI1 antibody (N-Term)

Catalog No. ABIN2780392

The Rabbit Polyclonal anti-FLI1 antibody has been validated for WB. It is suitable to detect FLI1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). There is 1 publication available.

Quick Overview for FLI1 antibody (N-Term) (ABIN2780392)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FLI1 Antibody

Binding Specificity: N-Term

Sequence: MDGTIKEALS VVSDDQSLFD SAYGAAAHLP KADMTASGSP DYGQPHKINP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against FLI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FLI1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 452 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FLI1 antibody (ABIN2780392)

Bujor, Haines, Padilla, Christmann, Junie, Sampaio-Barros, Lafyatis, Trojanowska: "Ciprofloxacin has antifibrotic effects in scleroderma fibroblasts via downregulation of Dnmt1 and upregulation of Fli1." in: International journal of molecular medicine, Vol. 30, Issue 6, pp. 1473-80, (2012) (PubMed).

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: FLI1

Background: Friend leukemia integration 1 (Fli-1) is a member of the ETS family of transcriptional regulatory proteins that contain a highly conserved and structurally unique DNA binding ETS domain.
Alias Symbols: EWSR2, SIC-1
Protein Interaction Partner: SOX30, SIAH1, CTBP2, UBC, SMAD9, GATA1, FMNL1, SUMO1, UBE2I, PIAS2, PIAS1, SMAD3, ETV6, KLF1, TDP2, SRF, ERG, EWSR1, ELK1, BRCA1, TGFBR2, STYXL1,
Protein Size: 452

Molecular Weight: 51 kDa

Gene ID: 2313

NCBI Accession: NM_002017, NP_002008

UniProt: Q01543