Phospholipase D2 antibody (N-Term)

Catalog No. ABIN2780445

The Rabbit Polyclonal anti-Phospholipase D2 antibody has been validated for WB. It is suitable to detect Phospholipase D2 in samples from Human, Rat, Mouse, Cow, Horse, Dog, Rabbit and Guinea Pig.

Quick Overview for Phospholipase D2 antibody (N-Term) (ABIN2780445)

Target: Phospholipase D2 (PLD2)

Reactivity: Human, Rat, Mouse, Cow, Horse, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase D2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Phospholipase D2 Antibody

Binding Specificity: N-Term

Sequence: MTATPESLFP TGDELDSSQL QMESDEVDTL KEGEDPADRM HPFLAIYELQ

Predicted Reactivity: Cow: 86%, Dog: 85%, Guinea Pig: 83%, Horse: 86%, Human: 100%, Mouse: 75%, Rabbit: 86%, Rat: 75%

Characteristics: This is a rabbit polyclonal antibody against PLD2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PLD2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 933 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Phospholipase D2

Target: Phospholipase D2 (PLD2)

Alternative Name: PLD2

Background: Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-14 AF033850.1 61-74 15-614 BC015033.1 1-600 615-1279 BC056871.1 549-1213 1280-1830 BC015033.1 1266-1816 1831-1995 BC056871.1 1765-1929 1996-3489 BC015033.1 1982-3475
Alias Symbols: -
Protein Interaction Partner: UBC, RPS6KB1, CUL3, AQP3, RPTOR, PEA15, PIP5K1A, STXBP1, DPYSL2, BIN1, MTOR, CAV1, AMPH, OPRM1, ALDOC, GM2A, PRKCD, PRKCA, PLCG1, LCK, GAPDH, FYN, EGFR, CSK, ALDOA, ARF1, ACTB, ACTN1, CAV2, SRC,
Protein Size: 933

Molecular Weight: 106 kDa

Gene ID: 5338

NCBI Accession: NM_002663, NP_002654

UniProt: O14939

Pathways: RTK Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling