EDF1 antibody (Middle Region)

Catalog No. ABIN2780547

This anti-EDF1 antibody is a Rabbit Polyclonal antibody detecting EDF1 in WB, IHC and IF. Suitable for Human, Mouse, Rat, Dog, Cow, Guinea Pig and Horse.

Quick Overview for EDF1 antibody (Middle Region) (ABIN2780547)

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EDF1 Antibody

Binding Specificity: Middle Region

Sequence: INEKPQVIAD YESGRAIPNN QVLGKIERAI GLKLRGKDIG KPIEKGPRAK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EDF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EDF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 148 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EDF1

Target: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Alternative Name: EDF1

Background: EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene. This gene encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.
Alias Symbols: EDF-1, MBF1, MGC9058
Protein Interaction Partner: MEOX2, UBC, IDH3G, UBE2I, MAPK6, NRL, JUN, CEBPA, NR1H3, NR5A2, PPARG, TBP, TAF1, NR5A1, RXRA, PRKCA, ATF2, CREB1, ATF1, CALM3, CALM1,
Protein Size: 148

Molecular Weight: 16 kDa

Gene ID: 8721

NCBI Accession: NM_003792, NP_003783

UniProt: O60869