TIMELESS antibody (C-Term)

Catalog No. ABIN2780562

Product Details anti-TIMELESS Antibody

Target: TIMELESS (Timeless Homolog (TIMELESS))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Sheep, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMELESS antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EEEDAVGKEP LKAAPKKRQL LDSDEEQEED EGRNRAPELG APGIQKKKRY

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 83%, Horse: 83%, Human: 100%, Mouse: 90%, Rat: 100%, Sheep: 100%, Yeast: 91%

Characteristics: This is a rabbit polyclonal antibody against TIMELESS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TIMELESS

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1208 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TIMELESS

Target: TIMELESS (Timeless Homolog (TIMELESS))

Alternative Name: TIMELESS (TIMELESS Products)

Synonyms: TIM antibody, TIM1 antibody, hTIM antibody, C77407 antibody, Debt69 antibody, tim antibody, CG3234 antibody, Dmel\\CG3234 antibody, Ritsu antibody, Tim antibody, dTIM antibody, dTim antibody, dtim antibody, mel_tim antibody, rit antibody, tim1 antibody, XTimeless1 antibody, timeless antibody, timelessb antibody, TIMELESS antibody, MXC20.14 antibody, MXC20_14 antibody, htim antibody, im:6903167 antibody, wu:fa95g04 antibody, wu:fd23d07 antibody, timeless circadian regulator antibody, timeless circadian clock 1 antibody, timeless antibody, timeless circadian clock antibody, timeless circadian clock S homeolog antibody, timeless family protein antibody, TIMELESS antibody, Timeless antibody, tim antibody, timeless.S antibody, Tim antibody, ATIM antibody, timeless antibody, LOC100328917 antibody

Background: The human Timeless protein interacts with both the circadian clock protein cryptochrome 2 and with the cell cycle checkpoint proteins Chk1 and the ATR-ATRIP complex and plays an important role in the DNA damage checkpoint response. Down-regulation of Timeless in human cells seriously compromises replication and intra-S checkpoints, indicating an intimate connection between the circadian cycle and the DNA damage checkpoints that is in part mediated by the Timeless protein.
Alias Symbols: TIM, TIM1, hTIM
Protein Interaction Partner: UBC, TUT1, TF, XRCC1, FKBP3, SP1, SUMO2, Tipin, Timeless, ZSCAN1, PCBD1, CRY1, PER2, PER1, CLSPN, PCNA, per, Clock, ATRIP, CRY2, CHEK1,
Protein Size: 1208

Molecular Weight: 139 kDa

Gene ID: 8914

NCBI Accession: NM_003920, NP_003911

UniProt: Q9UNS1

Pathways: Protein targeting to Nucleus, Photoperiodism