MED7 antibody (C-Term)

Catalog No. ABIN2780584

This Rabbit Polyclonal antibody specifically detects MED7 in WB and ChIP. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig and Horse.

Quick Overview for MED7 antibody (C-Term) (ABIN2780584)

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED7 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-MED7 Antibody

Binding Specificity: C-Term

Purpose: MED7 Antibody - C-terminal region

Sequence: LASLPDDLPH SEAGMRVKTE PMDADDSNNC TGQNEHQREN SGHRRDQIIE

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MED7. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38446-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED7

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Alternative Name: MED7

Background:

Targeted Subunit: 7

Background Information: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.

Gene Name: Mediator complex subunit 7

Alternative Symbols: ARC34, CRSP9, CRSP33

Protein Name: Mediator of RNA polymerase II transcription subunit 7

Molecular Weight: 27kDa

Gene ID: 9443

NCBI Accession: NP_004261

UniProt: O43513

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha