RNF14 antibody (C-Term)

Catalog No. ABIN2780586

This Rabbit Polyclonal antibody specifically detects RNF14 in WB and IHC. It exhibits reactivity toward Human, Rat, Cow, Dog and Rabbit.It has been mentioned in 1 publication

Quick Overview for RNF14 antibody (C-Term) (ABIN2780586)

Target: RNF14 (Ring Finger Protein 14 (RNF14))

Reactivity: Human, Rat, Cow, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RNF14 Antibody

Binding Specificity: C-Term

Purpose: RNF14 Antibody - C-terminal region

Sequence: CMQYFCWICM GSLSRANPYK HFNDPGSPCF NRLFYAVDVD DDIWEDEVED

Predicted Reactivity: Cow: 79%, Dog: 79%, Human: 100%, Rabbit: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against RNF14. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RNF14

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8110821

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RNF14 antibody (ABIN2780586)

Lin, Wu, Tarr, Lindenberg, St-Pierre, Zhang, Mootha, Jäger, Vianna, Reznick, Cui, Manieri, Donovan, Wu, Cooper, Fan, Rohas, Zavacki, Cinti, Shulman, Lowell, Krainc, Spiegelman: "Defects in adaptive energy metabolism with CNS-linked hyperactivity in PGC-1alpha null mice." in: Cell, Vol. 119, Issue 1, pp. 121-35, (2004) (PubMed).

Target Details for RNF14

Target: RNF14 (Ring Finger Protein 14 (RNF14))

Alternative Name: RNF14

Background:

Background Information: RNF14 contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins.The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Gene Name: Ring finger protein 14

Alternative Symbols: ARA54, HFB30, TRIAD2, HRIHFB2038

Protein Name: E3 ubiquitin-protein ligase RNF14

Molecular Weight: 52kDa

Gene ID: 9604

NCBI Accession: NP_899648

UniProt: Q9UBS8

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling