ONECUT1 antibody (Middle Region)

Catalog No. ABIN2780599

Cited in 2+ publications. The Rabbit Polyclonal anti-ONECUT1 antibody (ABIN2780599) specifically detects ONECUT1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Cow, Pig, Horse and Zebrafish (Danio rerio) samples.

Quick Overview for ONECUT1 antibody (Middle Region) (ABIN2780599)

Target: ONECUT1 (One Cut Homeobox 1 (ONECUT1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ONECUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ONECUT1 Antibody

Binding Specificity: Middle Region

Sequence: ITISQQLGLE LSTVSNFFMN ARRRSLDKWQ DEGSSNSGNS SSSSSTCTKA

Predicted Reactivity: Cow: 93%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ONECUT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ONECUT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 465 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ONECUT1 antibody (ABIN2780599)

Yuan, Wang, Hu, Tang, Shi, Guo, Song: "Hepatocyte nuclear factor 6 suppresses the migration and invasive growth of lung cancer cells through p53 and the inhibition of epithelial-mesenchymal transition." in: The Journal of biological chemistry, Vol. 288, Issue 43, pp. 31206-16, (2013) (PubMed).

Huang, Mei, Yu, Zhang, Han, Jiang, Zhou: "Distribution of the interstitial Cajal-like cells in the gallbladder and extrahepatic biliary duct of the guinea-pig." in: Acta histochemica, Vol. 111, Issue 2, pp. 157-65, (2009) (PubMed).

Target Details for ONECUT1

Target: ONECUT1 (One Cut Homeobox 1 (ONECUT1))

Alternative Name: ONECUT1

Background: ONECUT1 belongs to the CUT homeobox family. It contains 1 CUT DNA-binding domain and 1 homeobox DNA-binding domain. It is a transcriptional activator. ONECUT1 binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. It is important for liver genes transcription.
Alias Symbols: HNF-6, HNF6, HNF6A
Protein Interaction Partner: NR0B2, KAT2B, FOXA2, CREBBP, NR3C1, FOXA1,
Protein Size: 465

Molecular Weight: 51 kDa

Gene ID: 3175

NCBI Accession: NM_004498, NP_004489

UniProt: Q9UBC0