HDAC1 antibody (C-Term)

Catalog No. ABIN2780629

The Rabbit Polyclonal anti-HDAC1 antibody is suitable to detect HDAC1 in samples from Human, Mouse, Rat, Rabbit, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for HDAC1 antibody (C-Term) (ABIN2780629)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HDAC1 Antibody

Binding Specificity: C-Term

Purpose: HDAC1 Antibody - C-terminal region

Sequence: SDSEEEGEGG RKNSSNFKKA KRVKTEDEKE KDPEEKKEVT EEEKTKEEKP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Yeast: 91%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against HDAC1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HDAC1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8102585

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC1

Background:

Background Information: Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. HDAC1 also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Gene Name: Histone deacetylase 1

Alternative Symbols: HD1, RPD3, KDAC1, GON-10, RPD3L1

Protein Name: Histone deacetylase 1

Molecular Weight: 55kDa

Gene ID: 3065

NCBI Accession: NP_004955

UniProt: Q13547

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis