MECP2 antibody (Middle Region)

Catalog No. ABIN2780632

This Rabbit Polyclonal antibody specifically detects MECP2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Horse, Dog, Cow, Guinea Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for MECP2 antibody (Middle Region) (ABIN2780632)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Human, Mouse, Rat, Horse, Dog, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MECP2 Antibody

Binding Specificity: Middle Region

Sequence: EPAKTQPAVA TAATAAEKYK HRGEGERKDI VSSSMPRPNR EEPVDSRTPV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against MECP2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MECP2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 486 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MECP2

Background: Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Alias Symbols: AUTSX3, DKFZp686A24160, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT, RS
Protein Interaction Partner: UBC, HECW2, Hipk2, SOX18, WHSC1, HPDL, XPC, CAT, HIST2H3C, HIST1H4F, HIST2H2BE, HIST2H2AC, CBX3, CBX1, SMC3, YBX1, SIN3A, CBX5, ELAVL1, SUMO2, PPARG, YY1, TERF2, SMARCA2, SOX2, SP1, SP3, LOC407840, CDKL5, PRPF40A, SMARCB1, NCOR1, SKI, HMGB1, SPI1, LBR, RB
Protein Size: 486

Molecular Weight: 52 kDa

Gene ID: 4204

NCBI Accession: NM_004992, NP_004983

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane