NRF1 antibody (C-Term)

Catalog No. ABIN2780636

The Rabbit Polyclonal anti-NRF1 antibody is suitable to detect NRF1 in samples from Human and Rat. It has been validated for WB and IHC.

Quick Overview for NRF1 antibody (C-Term) (ABIN2780636)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NRF1 Antibody

Binding Specificity: C-Term

Purpose: NRF1 Antibody - C-terminal region

Sequence: IVLSGETAAA VGALTGVQDA NGLFMADRAG RKWILTDKAT GLVQIPVSMY

Predicted Reactivity: Human: 100%, Rat: 91%

Characteristics: This is a rabbit polyclonal antibody against NRF1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NRF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8106872

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF1

Background:

Background Information: NRF1 is a phosphorylated nuclear protein with a bZIP domain. This protein homodimerizes and functions as a transcription factor that activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth.This gene encodes a phosphorylated nuclear protein with a bZIP domain. This protein homodimerizes and functions as a transcription factor that activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized.

Gene Name: Nuclear respiratory factor 1

Alternative Symbols: ALPHA-PAL

Protein Name: NRF1 protein EMBL AAH16925.1

Molecular Weight: 56kDa

Gene ID: 4899

NCBI Accession: NP_005002

UniProt: Q96AN2

Pathways: Regulation of Lipid Metabolism by PPARalpha