AFT1 antibody (N-Term)

Catalog No. ABIN2780653

This anti-AFT1 antibody is a Rabbit Polyclonal antibody detecting AFT1 in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig and Sheep.

Quick Overview for AFT1 antibody (N-Term) (ABIN2780653)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AFT1 Antibody

Binding Specificity: N-Term

Sequence: ETAPQPGSAV QGAHISHIAQ QVSSLSESEE SQDSSDSIGS SQKAHGILAR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 85%

Characteristics: This is a rabbit polyclonal antibody against ATF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 271 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Alternative Name: ATF1

Background: ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT [AC] [AG]-3'), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.
Alias Symbols: TREB36, EWS-ATF1, FUS/ATF-1
Protein Interaction Partner: UBC, SRA1, FOSL2, GABPB1, GABPA, EDF1, HNF1B, RPS6KA5, CAMK1, RPS6KA4, CAMK2G, PRKACA, CREBBP, SPI1, JUN, CREM, CREB1, ATF1, CSNK2A2, CSNK2A1, BRCA1, CSNK2B, CAMK2A, FOSL1,
Protein Size: 271

Molecular Weight: 29 kDa

Gene ID: 466

NCBI Accession: NM_005171, NP_005162

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades