SMAD4 antibody (N-Term)

Catalog No. ABIN2780673

This Rabbit Polyclonal antibody specifically detects SMAD4 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Goat, Guinea Pig, Horse and Sheep.

Quick Overview for SMAD4 antibody (N-Term) (ABIN2780673)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Goat, Guinea Pig, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SMAD4 Antibody

Binding Specificity: N-Term

Sequence: MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SMAD4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 552 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantl
Alias Symbols: DPC4, JIP, MADH4, MYHRS
Protein Interaction Partner: RASSF5, LMO4, SMAD3, SMAD2, SKIL, UBC, NEDD8, SUMO1, SMURF2, SMURF1, TRIM33, CRYAB, COPS5, NLK, OTUB1, SMAD9, UBE2I, FHL3, FHL2, FHL1, CSNK1D, TGFB1I1, SMAD1, NCOR1, SKI, KLF5, IRF7, IRF3, ZNF521, ZNF423, SOX12, SNRNP70, RPL28, RFX1, RELA, RALA, PSMD11, P
Protein Size: 552

Molecular Weight: 60 kDa

Gene ID: 4089

NCBI Accession: NM_005359, NP_005350

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy