NFIL3 antibody (N-Term)

Catalog No. ABIN2780681

The Rabbit Polyclonal anti-NFIL3 antibody has been validated for WB and IHC. It is suitable to detect NFIL3 in samples from Human, Cow, Sheep, Dog and Pig.

Quick Overview for NFIL3 antibody (N-Term) (ABIN2780681)

Target: NFIL3 (Nuclear Factor, Interleukin 3 Regulated (NFIL3))

Reactivity: Human, Cow, Sheep, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NFIL3 Antibody

Binding Specificity: N-Term

Sequence: MQLRKMQTVK KEQASLDASS NVDKMMVLNS ALTEVSEDST TGEDVLLSEG

Predicted Reactivity: Cow: 86%, Dog: 86%, Human: 100%, Pig: 79%, Sheep: 79%

Characteristics: This is a rabbit polyclonal antibody against NFIL3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NFIL3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 462 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFIL3

Target: NFIL3 (Nuclear Factor, Interleukin 3 Regulated (NFIL3))

Alternative Name: NFIL3

Background: NFIL3 is a basic leucine zipper (bZIP) transcription factor that represses or activates transcription in non-osteoblastic cells. NFIL3 play a role in attenuation of PTH target gene transcription in osteoblasts. NFIL3 repression domain interacts specifically with the TBP binding repressor protein Dr1.Glucocorticoid-mediated upregulation of the bZIP transcriptional repressor gene, NFIL3, is dependent on [Ca2+]i levels, and correlates with Glucocorticoid-evoked apoptosis of Glucocorticoid-sensitive CEM-C7-14 cells. NFIL3 is involved in a distinct growth factor-regulated signaling pathway that is responsible for the survival of early B-cell progenitors, and whose alteration by E2A-HLF leads to childhood B lineage leukemia.
Alias Symbols: E4BP4, IL3BP1, NFIL3A, NF-IL3A
Protein Interaction Partner: AMOTL2, TRAF2, MAFF, BATF, NFIL3, MAFG, DDIT3, CBX8, SUMO2, UBC, CREB3, DR1,
Protein Size: 462

Molecular Weight: 51 kDa

Gene ID: 4783

NCBI Accession: NM_005384, NP_005375

UniProt: Q14211