NR1H3 antibody (C-Term)

Catalog No. ABIN2780709

There is 1 publication for this product available. The Rabbit Polyclonal anti-NR1H3 antibody is suitable to detect NR1H3 in samples from Human, Mouse, Rat, Cow, Goat, Dog, Horse, Rabbit, Guinea Pig and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for NR1H3 antibody (C-Term) (ABIN2780709)

Target: NR1H3 (Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3))

Reactivity: Human, Mouse, Rat, Cow, Goat, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR1H3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NR1H3 Antibody

Binding Specificity: C-Term

Purpose: NR1H3 Antibody - C-terminal region

Sequence: IHHPHDRLMF PRMLMKLVSL RTLSSVHSEQ VFALRLQDKK LPPLLSEIWD

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against NR1H3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NR1H3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38670-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NR1H3 antibody (ABIN2780709)

Torra, Ismaili, Feig, Xu, Cavasotto, Pancratov, Rogatsky, Neubert, Fisher, Garabedian: "Phosphorylation of liver X receptor alpha selectively regulates target gene expression in macrophages." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2626-36, (2008) (PubMed).

Target Details for NR1H3

Target: NR1H3 (Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3))

Alternative Name: NR1H3

Background:

Background Information: Interaction of NR1H3 with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. NR1H3 are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. NR1H3 Plays an important role in the regulation of cholesterol homeostasis.

Gene Name: Nuclear receptor subfamily 1, group H, member 3

Alternative Symbols: LXRA, LXR-a, RLD-1

Protein Name: Oxysterols receptor LXR-alpha

Molecular Weight: 50 kDa

Gene ID: 10062

NCBI Accession: NP_005684

UniProt: Q13133

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Nuclear Hormone Receptor Binding, Cellular Response to Molecule of Bacterial Origin, Hepatitis C