KAP1 antibody (C-Term)

Catalog No. ABIN2780714

The Rabbit Polyclonal anti-KAP1 antibody has been validated for WB and IHC. It is suitable to detect KAP1 in samples from Human, Mouse, Rat, Rabbit, Dog, Cow, Horse and Guinea Pig.

Quick Overview for KAP1 antibody (C-Term) (ABIN2780714)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-KAP1 Antibody

Binding Specificity: C-Term

Sequence: QFNKLTEDKA DVQSIIGLQR FFETRMNEAF GDTKFSAVLV EPPPMSLPGA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TRIM28. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TRIM28

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 835 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TRIM28

Background: TRIM28 mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. TRIM28 localizes to the nucleus and is thought to associate with specific chromatin regions. It is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING,a B-box type 1 and a B-box type 2, and a coiled-coil region.
Alias Symbols: KAP1, TF1B, RNF96, TIF1B
Protein Interaction Partner: WWOX, ERLIN2, HUWE1, SUMO1, UBC, SPRTN, SENP7, HAUS2, MAGEC2, CBX5, WHSC1, UBE2I, AURKA, SUMO2, SUMO3, RNF4, CEP250, TRIM28, HIF1A, CHD3, ATM, ZNF350, STAU1, HIST3H3, LYN, NEDD8, MDM2, ZBTB1, RPA3, RPA2, RPA1, RNF2, SUZ12, EZH2, BMI1, EED, BRCC3, WRNIP1,
Protein Size: 835

Molecular Weight: 89 kDa

Gene ID: 10155

NCBI Accession: NM_005762, NP_005753

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus