HIC1 antibody (Middle Region)

Catalog No. ABIN2780783

There is 1 publication for this product available. The Rabbit Polyclonal anti-HIC1 antibody is suitable to detect HIC1 in samples from Human, Rat, Mouse, Guinea Pig, Dog, Cow and Horse. It has been validated for WB.

Quick Overview for HIC1 antibody (Middle Region) (ABIN2780783)

Target: HIC1 (Hypermethylated in Cancer 1 (HIC1))

Reactivity: Human, Rat, Mouse, Guinea Pig, Dog, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HIC1 Antibody

Binding Specificity: Middle Region

Purpose: HIC1 Antibody - middle region

Sequence: GETPIAVMGE FANLATSLNP LDPDKDEEEE EEEESEDELP QDISLAAGGE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against HIC1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HIC1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38806-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HIC1 antibody (ABIN2780783)

Stöcklein, Smardova, Macak, Katzenberger, Höller, Wessendorf, Hutter, Dreyling, Haralambieva, Mäder, Müller-Hermelink, Rosenwald, Ott, Kalla: "Detailed mapping of chromosome 17p deletions reveals HIC1 as a novel tumor suppressor gene candidate telomeric to TP53 in diffuse large B-cell lymphoma." in: Oncogene, Vol. 27, Issue 18, pp. 2613-25, (2008) (PubMed).

Target Details for HIC1

Target: HIC1 (Hypermethylated in Cancer 1 (HIC1))

Alternative Name: HIC1

Background:

Background Information: HIC1 is a transcriptional repressor. HIC1 may act as a tumor suppressor. HIC1 may be involved in development of head, face, limbs and ventral body wall. Defects in HIC1 may be a cause of the facial dysmorphism and additional birth defects (except for lissencephaly) seen in the contiguous gene disorder Miller-Dieker syndrome (MDS), like defective development of the nose, jaws, extremities, gastrointestinal tract, and kidney.

Gene Name: Hypermethylated in cancer 1

Alternative Symbols: hic-1, ZBTB29, ZNF901

Protein Name: Hypermethylated in cancer 1 protein

Molecular Weight: 74kDa

Gene ID: 3090

NCBI Accession: NP_006488

UniProt: Q14526

Pathways: Positive Regulation of Response to DNA Damage Stimulus