SUPT16H antibody (Middle Region)

Catalog No. ABIN2780836

The Rabbit Polyclonal anti-SUPT16H antibody (ABIN2780836) specifically detects SUPT16H in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Zebrafish (Danio rerio) and Goat samples.

Quick Overview for SUPT16H antibody (Middle Region) (ABIN2780836)

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT16H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SUPT16H Antibody

Binding Specificity: Middle Region

Purpose: SUPT16H Antibody - middle region

Sequence: EESDYSKESL GSEEESGKDW DELEEEARKA DRESRYEEEE EQSRSMSRKR

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against SUPT16H. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SUPT16H

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38921-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SUPT16H

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Alternative Name: SUPT16H

Background:

Targeted Subunit: SPT16

Background Information: Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is SUPT16H. Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene. Sequence Note: The sequence AF152961.1 is a chimeric mRNA clone. Only the SUPT16H region was propagated into this RefSeq record. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Suppressor of Ty 16 homolog (S. cerevisiae)

Alternative Symbols: CDC68, SPT16, FACTP140, SPT16/CDC68

Protein Name: FACT complex subunit SPT16

Molecular Weight: 120kDa

Gene ID: 11198

NCBI Accession: NP_009123

UniProt: Q9Y5B9

Pathways: Chromatin Binding