IKZF3 antibody (C-Term)

Catalog No. ABIN2780887

The Rabbit Polyclonal anti-IKZF3 antibody (ABIN2780887) specifically detects IKZF3 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse and Zebrafish (Danio rerio) samples.

Quick Overview for IKZF3 antibody (C-Term) (ABIN2780887)

Target: IKZF3 (IKAROS Family Zinc Finger 3 (Aiolos) (IKZF3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKZF3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IKZF3 Antibody

Binding Specificity: C-Term

Purpose: IKZF3 Antibody - C-terminal region

Sequence: LDYVMFTIHM GCHGFRDPFE CNMCGYRSHD RYEFSSHIAR GEHRALLK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against IKZF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human IKZF3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39005-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for IKZF3

Target: IKZF3 (IKAROS Family Zinc Finger 3 (Aiolos) (IKZF3))

Alternative Name: IKZF3

Background:

Background Information: IKZF3 is a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoetic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described.This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described.

Gene Name: IKAROS family zinc finger 3 (Aiolos)

Alternative Symbols: AIO, AIOLOS, ZNFN1A3

Protein Name: Zinc finger protein Aiolos

Molecular Weight: 58kDa

Gene ID: 22806

NCBI Accession: NP_036613

UniProt: Q9UKT9