PATZ1 antibody (Middle Region)

Catalog No. ABIN2780923

The Rabbit Polyclonal anti-PATZ1 antibody (ABIN2780923) specifically detects PATZ1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse and Zebrafish (Danio rerio) samples.

Quick Overview for PATZ1 antibody (Middle Region) (ABIN2780923)

Target: PATZ1 (ZNF278) (Zinc Finger Protein 278 (ZNF278))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PATZ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PATZ1 Antibody

Binding Specificity: Middle Region

Purpose: PATZ1 Antibody - middle region

Sequence: SFFRSKSYLN KHIQKVHVRA LGGPLGDLGP ALGSPFSPQQ NMSLLESFGF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against PATZ1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PATZ1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39078-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PATZ1

Target: PATZ1 (ZNF278) (Zinc Finger Protein 278 (ZNF278))

Alternative Name: PATZ1

Background:

Background Information: PATZ1 contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1,22)(p36.1,q12).The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1,22)(p36.1,q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene.

Gene Name: POZ (BTB) and AT hook containing zinc finger 1

Alternative Symbols: ZSG, MAZR, PATZ, RIAZ, ZBTB19, ZNF278, dJ400N23

Protein Name: POZ-, AT hook-, and zinc finger-containing protein 1

Molecular Weight: 74kDa

Gene ID: 23598

NCBI Accession: NP_055138

UniProt: Q9HBE1