SART3 antibody (N-Term)

Catalog No. ABIN2780944

This Rabbit Polyclonal antibody specifically detects SART3 in WB and IHC. It exhibits reactivity toward Human, Rat, Guinea Pig, Dog, Horse and Pig.It has been mentioned in 1 publication

Quick Overview for SART3 antibody (N-Term) (ABIN2780944)

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Reactivity: Human, Rat, Guinea Pig, Dog, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SART3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SART3 Antibody

Binding Specificity: N-Term

Purpose: SART3 Antibody - N-terminal region

Sequence: TSASEPEAES KAGPKADGEE DEVKAARTRR KVLSRAVAAA TYKTMGPAWD

Predicted Reactivity: Dog: 77%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Pig: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against SART3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SART3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39128-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SART3 antibody (ABIN2780944)

Kaji, Mizukoshi, Yamashita, Arai, Sunagozaka, Fushimi, Nakagawa, Yamada, Terashima, Kitahara, Kaneko: "Cellular Immune Responses for Squamous Cell Carcinoma Antigen Recognized by T Cells 3 in Patients with Hepatocellular Carcinoma." in: PLoS ONE, Vol. 12, Issue 1, pp. e0170291, (2017) (PubMed).

Target Details for SART3

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Alternative Name: SART3

Background:

Background Information: SART3 is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. SART3 is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This protein is thought to be involved in the regulation of mRNA splicing.

Gene Name: Squamous cell carcinoma antigen recognized by T cells 3

Alternative Symbols: P100, p110, DSAP1, TIP110, p110(nrb), RP11-13G14

Protein Name: Squamous cell carcinoma antigen recognized by T-cells 3

Molecular Weight: 40kDa

Gene ID: 9733

UniProt: Q15020

Pathways: Ribonucleoprotein Complex Subunit Organization