BCL11A antibody (N-Term)

Catalog No. ABIN2781048

There is 1 publication for this product available. The Rabbit Polyclonal anti-BCL11A antibody is suitable to detect BCL11A in samples from Human, Mouse, Rat, Cow, Dog, Horse, Pig and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for BCL11A antibody (N-Term) (ABIN2781048)

Target: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL11A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BCL11A Antibody

Binding Specificity: N-Term

Purpose: BCL11A Antibody - N-terminal region

Sequence: MSRRKQGKPQ HLSKREFSPE PLEAILTDDE PDHGPLGAPE GDHDLLTCGQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against BCL11A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BCL11A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39322-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-BCL11A antibody (ABIN2781048)

Uda, Galanello, Sanna, Lettre, Sankaran, Chen, Usala, Busonero, Maschio, Albai, Piras, Sestu, Lai, Dei, Mulas, Crisponi, Naitza, Asunis, Deiana, Nagaraja, Perseu, Satta, Cipollina, Sollaino, Moi, Hirschhorn, Orkin, Abecasis, Schlessinger, Cao: "Genome-wide association study shows BCL11A associated with persistent fetal hemoglobin and amelioration of the phenotype of beta-thalassemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 5, pp. 1620-5, (2008) (PubMed).

Target Details for BCL11A

Target: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Alternative Name: BCL11A

Background:

Background Information: BCL11A Is a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression.This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene.

Gene Name: B-cell CLL/lymphoma 11A (zinc finger protein)

Alternative Symbols: EVI9, CTIP1, DILOS, ZNF856, HBFQTL5, BCL11A-L, BCL11A-S, BCL11a-M, BCL11A-XL

Protein Name: B-cell lymphoma/leukemia 11A

Molecular Weight: 91kDa

Gene ID: 53335

NCBI Accession: NP_075044

UniProt: Q9H165

Pathways: Regulation of Cell Size