KDM6A antibody (C-Term)

Catalog No. ABIN2781142

The Rabbit Polyclonal anti-KDM6A antibody (ABIN2781142) specifically detects KDM6A in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse and Rabbit samples.

Quick Overview for KDM6A antibody (C-Term) (ABIN2781142)

Target: KDM6A (Lysine (K)-Specific Demethylase 6A (KDM6A))

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM6A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KDM6A Antibody

Binding Specificity: C-Term

Purpose: UTX Antibody - C-terminal region

Sequence: KTYIVHCQDC ARKTSGNLEN FVVLEQYKME DLMQVYDQFT LAPPLPSASS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against UTX. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human UTX

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39483-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for KDM6A

Target: KDM6A (Lysine (K)-Specific Demethylase 6A (KDM6A))

Alternative Name: UTX

Background:

Background Information: UTX is a histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. It demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. UTX plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.

Gene Name: Lysine (K)-specific demethylase 6A

Alternative Symbols: UTX, KABUK2, bA386N14.2

Protein Name: Lysine-specific demethylase 6A

Molecular Weight: 154kDa

Gene ID: 7403

NCBI Accession: NP_066963

UniProt: O15550

Pathways: Tube Formation, Warburg Effect