GRHL3 antibody (C-Term)

Catalog No. ABIN2781145

There is 1 publication for this product available. The Rabbit Polyclonal anti-GRHL3 antibody is suitable to detect GRHL3 in samples from Human, Mouse, Dog, Rat, Cow, Guinea Pig, Horse, Rabbit and Pig. It has been validated for WB, IHC and ChIP.

Quick Overview for GRHL3 antibody (C-Term) (ABIN2781145)

Target: GRHL3 (Grainyhead-Like 3 (GRHL3))

Reactivity: Human, Mouse, Dog, Rat, Cow, Guinea Pig, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRHL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)

Product Details anti-GRHL3 Antibody

Binding Specificity: C-Term

Purpose: GRHL3 Antibody - C-terminal region

Sequence: EEVFDALMLK TPDLKGLRNA ISEKYGFPEE NIYKVYKKCK RGILVNMDNN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against GRHL3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GRHL3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8101887

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GRHL3 antibody (ABIN2781145)

Darido, Georgy, Wilanowski, Dworkin, Auden, Zhao, Rank, Srivastava, Finlay, Papenfuss, Pandolfi, Pearson, Jane: "Targeting of the tumor suppressor GRHL3 by a miR-21-dependent proto-oncogenic network results in PTEN loss and tumorigenesis." in: Cancer cell, Vol. 20, Issue 5, pp. 635-48, (2011) (PubMed).

Target Details for GRHL3

Target: GRHL3 (Grainyhead-Like 3 (GRHL3))

Alternative Name: GRHL3

Background:

Background Information: GRHL3 is a member of the grainyhead family of transcription factors. GRHL3 interacts with leader-binding protein 32 (LBP-32) and brother of mammalian grainyhead (BOM), and may function as a transcription factor during development. Multiple transcript variants encoding distinct isoforms have been identified for this gene. This gene encodes a member of the grainyhead family of transcription factors. The encoded protein interacts with leader-binding protein 32 (LBP-32) and brother of mammalian grainyhead (BOM), and may function as a transcription factor during development. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional transcript variants have been described, but their biological nature has not been determined.

Gene Name: Grainyhead-like 3 (Drosophila)

Alternative Symbols: SOM, VWS2, TFCP2L4

Protein Name: Grainyhead-like protein 3 homolog

Molecular Weight: 57 kDa, 70 kDa

Gene ID: 57822

NCBI Accession: NP_937817

UniProt: Q8TE85