AADAT antibody (N-Term)

Catalog No. ABIN2781307

The Rabbit Polyclonal anti-AADAT antibody (ABIN2781307) specifically detects AADAT in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Rabbit, Horse, Cow, Dog, Zebrafish (Danio rerio) and Pig samples.

Quick Overview for AADAT antibody (N-Term) (ABIN2781307)

Target: AADAT (Aminoadipate Aminotransferase (AADAT))

Reactivity: Human, Mouse, Rat, Rabbit, Horse, Cow, Dog, Zebrafish (Danio rerio), Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AADAT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AADAT Antibody

Binding Specificity: N-Term

Purpose: AADAT Antibody - N-terminal region

Sequence: AVITVENGKT IQFGEEMMKR ALQYSPSAGI PELLSWLKQL QIKLHNPPTI

Predicted Reactivity: Cow: 93%, Dog: 93%, Horse: 86%, Human: 100%, Mouse: 93%, Pig: 79%, Rabbit: 100%, Rat: 85%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against AADAT. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AADAT

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43534-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for AADAT

Target: AADAT (Aminoadipate Aminotransferase (AADAT))

Alternative Name: AADAT

Background:

Background Information: AADAT is a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties.This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist.

Gene Name: Aminoadipate aminotransferase

Alternative Symbols: KAT2, KATII, KYAT2

Protein Name: Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial

Molecular Weight: 47 kDa

Gene ID: 51166

NCBI Accession: NP_057312

UniProt: Q8N5Z0