GCDH antibody (N-Term)

Catalog No. ABIN2781324

The Rabbit Polyclonal anti-GCDH antibody has been validated for WB. It is suitable to detect GCDH in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Rabbit, Horse, Cow and Guinea Pig.

Quick Overview for GCDH antibody (N-Term) (ABIN2781324)

Target: GCDH (Glutaryl-CoA Dehydrogenase (GCDH))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Rabbit, Horse, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCDH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GCDH Antibody

Binding Specificity: N-Term

Sequence: SLVMHPIYAY GSEEQRQKYL PQLAKGELLG CFGLTEPNSG SDPSSMETRA

Predicted Reactivity: Cow: 92%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against GCDH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GCDH

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 438 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GCDH

Target: GCDH (Glutaryl-CoA Dehydrogenase (GCDH))

Alternative Name: GCDH

Background: GCDH belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits.Alternatively spliced transcript variants encoding different isoforms have been identified.The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alias Symbols: ACAD5, GCD
Protein Interaction Partner: UBC, FBXO6, ADRB2, RNF32, EXOC6, ECSIT, MAST1, CDC37, PSEN2, PSEN1, NOS3, APOE, A2M, UBD, MYC, GCDH,
Protein Size: 438

Molecular Weight: 43 kDa

Gene ID: 2639

NCBI Accession: NM_000159, NP_000150

UniProt: Q92947